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Linlin LIANG, Bei XU, Guijin ZHU
《医学前沿(英文)》 2009年 第3卷 第2期 页码 220-226 doi: 10.1007/s11684-009-0037-2
关键词: gonadotropin-releasing hormone ovarian reserve embryo developmental ability ovarian surface epithelium
null
《医学前沿(英文)》 2017年 第11卷 第2期 页码 247-252 doi: 10.1007/s11684-017-0519-6
The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IV embryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.
关键词: in vitro fertilization PCOS free radical embryo quality
Current techniques for assessing developmental neurotoxicity of pesticides
GAO Yu, TIAN Ying, SHEN Xiaoming
《医学前沿(英文)》 2008年 第2卷 第4期 页码 337-343 doi: 10.1007/s11684-008-0064-4
关键词: paradigms Pyrethroids non-mammalian susceptible morphological
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 373-381 doi: 10.15302/J-FASE-2018210
Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.
关键词: Aspergillus oryzae egg allergy egg white proteins IgE-binding ability ovomucoid
HIGH QUALITY DEVELOPMENTAL APPROACH FOR SOIL AND WATER CONSERVATION AND ECOLOGICAL PROTECTION ON THE
《农业科学与工程前沿(英文)》 2021年 第8卷 第4期 页码 501-511 doi: 10.15302/J-FASE-2021425
The Loess Plateau is the core area in the Yellow River basin for implementing environmental protection and high-quality development strategies. A series of ecological projects has implemented aimed at soil and water conservation and ecological management on the Loess Plateau over the past 70 years. The effects of the ecological projects are apparent mainly through a marked increase in vegetation cover, controlled soil erosion and reduced flow of sediment into the Yellow River, continual optimization of the industrial structure and increased production from arable land, poverty alleviation and greater prosperity, and optimal allocation of space for biological organisms. Major problems have also been analyzed in ecological management including the fragile ecosystem of the region, maintaining the stability of vegetation, lower agricultural productivity and continued risk from natural disasters. Some suitable schemes and models have been developed for the coordinated development of the region through research and demonstration, striking the optimum balance between rural industry and ecology, and increased regional capacity to supply high-quality ecological products. Countermeasures to address the problems are suggested to guide ecological management and high-quality development in the future.
关键词: ecological management high quality development industrial structure soil erosion soil and water conservation Loess Plateau
null
《医学前沿(英文)》 2017年 第11卷 第4期 页码 563-569 doi: 10.1007/s11684-017-0535-6
The superiority of the cumulative outcomes of day 5/6 embryo transfer to those of day 2/3 embryo transfer in infertile couples has been debated. This retrospective study included data collected from 1051 patients from July 2011 to June 2014. Multiple maternal baseline covariates were subjected to propensity score matching analysis, and each day 5/6 group woman was matched to one day 2/3 group woman. A systematic meta-analysis was conducted to validate the results. After matching was completed, 217 patients on the day 2/3 group were matched with those on the day 5/6 group, and no significant differences in the baseline characteristics were observed between the two groups. The cumulative pregnancy rate (57.14% vs. 53.46%, OR 1.16, 95% CI 0.79–1.70) and cumulative live birth rate (53.00% vs. 49.77%, OR 1.14, 95% CI 0.78–1.66) of day 5/6 embryo transfers were higher than those of day 2/3 embryo transfers, but this difference was not significant. The mean cycles per live birth and mean days per live birth in the day 5/6 group were significantly lower than those in the day 2/3 group. This study demonstrated that day 5/6 embryo transfer is a more cost-effective and time-efficient policy than day 2/3 embryo transfer to produce a live baby.
关键词: blastocyst embryo transfer cumulative pregnancy rate cumulative live birth rate IVF
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
《农业科学与工程前沿(英文)》 2020年 第7卷 第2期 页码 148-160 doi: 10.15302/J-FASE-2019305
Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.
关键词: animal breeding cattle cloning CRISPR/Cas9 cytoplasmic injection embryo genome editing germline chimaeras HDR livestock improvement TALENs
郭志勤
《中国工程科学》 2000年 第2卷 第3期 页码 12-17
从我国养牛业的现状和存在的主要问题,指出养牛业需要大力发展,当前必须大量引种和繁殖种牛。 而胚胎移植是引种的一种最好手段,具有方法简便、成本低、缩短改良时间、减少疾病传播等优点。同时,进 一步分析了我国胚胎移植技术的研究概况,阐明了胚胎移植的主要技术关键及产业化的必要性和可能性。并根 据我国养牛业的现状,提出奶牛、肉牛业应用胚胎移植技术的措施与办法。
解绶启,张文兵,韩冬,麦康森
《中国工程科学》 2016年 第18卷 第3期 页码 29-36 doi: 10.15302/J-SSCAE-2016.03.005
本文综述了我国水产养殖动物营养学研究和饲料工程方面近期的研究,分析了产业发展的问题,探讨了下一步的政策措施。可以发现,过去10多年里,水产动物营养与饲料的发展在营养素需求、动物的摄食与投喂、仔稚鱼饲料、亲本营养、添加剂等方面取得了重要的进展,但仍然存在饵料系数高、环境排放大、饲料成本高、效益不稳定等问题。下一步的研究应该以“安全高效,节约环保”为目标,引入生态学和现代组学理念,开展水产动物营养代谢的分子机理、动物的营养需要与精准饲料配方技术、饲料质量控制与加工工艺、精准投喂技术、品质调控技术等方面的研究,同时在政策上应该扩大学科交流、实行长期稳定支持等。
H. Watamura, H. Marukawa, I. Hirasawa
《化学科学与工程前沿(英文)》 2013年 第7卷 第1期 页码 55-59 doi: 10.1007/s11705-013-1312-y
刘忠波,唐军
《中国工程科学》 2010年 第12卷 第9期 页码 96-100
研究了一类含弱非线性的改进型Boussinesq水波方程,在非交错网格下,利用有限差分法建立了混合四阶Adams-Bashforth-Moulton的预报校正格式的波浪数值模型。在数值模型中,关于空间一阶导数差分格式采用四阶精度、二阶导数差分格式采用二阶精度。针对波浪的一维、二维传播变形问题进行了数值计算,并通过与相关实验结果对比分析考察了该数值模型的适用性。
曹光荣,胡亚安,高雄
《中国工程科学》 2007年 第9卷 第12期 页码 75-80
关键词: 三峡船闸;输水方式;水流条件;通过能力
Factors affecting early embryonic development in cattle: relevance for bovine cloning
Yanna DANG, Kun ZHANG
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 33-41 doi: 10.15302/J-FASE-2018228
Female infertility represents a major challenge for improving the production efficiency in the dairy industry. Historically, fertility has declined whereas milk yield has increased tremendously due to intensive genetic selection. evidence reveals about 60% pregnancy loss takes place during the first month following fertilization. Meanwhile, early embryo development is significant for somatic cell nuclear transfer in cattle as a large proportion of cloned embryos fail to develop beyond peri-implantation stage. Oocyte quality is of utmost importance for the early embryo to develop to term for both fertilized and cloned embryos. Epigenetic reprogramming is a key process occurring after fertilization and critical roles of epigenetic modifiers during preimplantation development are now clear. Incomplete epigenetic reprogramming is believed to be a major limitation to cloning efficiency. Treatment of cloned embryos with epigenetic modifying drugs (e.g., Trichostatin A) could greatly improve cloning efficiency in both mice and cattle. Recently, the rapid progress in high-throughput sequencing technologies has enabled detailed deciphering of the molecular mechanisms underlying these events. The robust efficiency of genomic editing tools also presents an alternative approach to the functional annotation of genes critical to early development.
关键词: bovine cloning embryo development somatic cell nuclear transfer X-inactive specific transcript
《化学科学与工程前沿(英文)》 2023年 第17卷 第6期 页码 735-748 doi: 10.1007/s11705-022-2262-z
关键词: overoxidized poly(3 4-ethylenedioxythiophene)-overoxidized polypyrrole rutin luteolin incubation electrocatalytic mechanism
Bisphenol-A epoxy resin reinforced and toughened by hyperbranched epoxy resin
ZHANG Daohong, JIA Demin, HUANG Xianbo
《化学科学与工程前沿(英文)》 2007年 第1卷 第4期 页码 349-354 doi: 10.1007/s11705-007-0063-z
关键词: thermogravimetric analysis developmental direction flexural strength HTDE-2/E51 technology
标题 作者 时间 类型 操作
Effect of repeated gonadotropin stimulation on ovarian reserves and proliferation of ovarian surface epithelium in mice
Linlin LIANG, Bei XU, Guijin ZHU
期刊论文
Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo
null
期刊论文
Current techniques for assessing developmental neurotoxicity of pesticides
GAO Yu, TIAN Ying, SHEN Xiaoming
期刊论文
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
期刊论文
HIGH QUALITY DEVELOPMENTAL APPROACH FOR SOIL AND WATER CONSERVATION AND ECOLOGICAL PROTECTION ON THE
期刊论文
Propensity score-matched study and meta-analysis of cumulative outcomes of day 2/3 versus day 5/6 embryo
null
期刊论文
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
期刊论文
Filtration ability of hollow fiber membrane for production of magnesium ammonium phosphate crystals by
H. Watamura, H. Marukawa, I. Hirasawa
期刊论文
Factors affecting early embryonic development in cattle: relevance for bovine cloning
Yanna DANG, Kun ZHANG
期刊论文
3,4-ethylenedioxythiophene)-overoxidized polypyrrole composite films with enhanced electrocatalytic ability
期刊论文